Efficient eye washing is advised after chemical exposure, considering the potential for irritation or corrosion even with natural protective mechanisms. Traditional eye irritation tests, like the in vivo rabbit eye test, are common for agrochemicals but face criticism, pushing for alternatives due to ethical concerns. The move towards animal-free testing aligns with directives aiming to end mammal testing by 2035. Despite existing in vitro/ex vivo methods, challenges remain in achieving concordance with in vivo results, especially for distinguishing mild and moderate irritants. Evaluating agrochemical formulations' complexity, including active and "inert" ingredients, highlights the need for human-relevant, reproducible alternatives, acknowledging differences between in vitro/ex vivo methods and the rabbit test.